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Essay Available:
Pages:
4 pages/≈1100 words
Sources:
Check Instructions
Style:
APA
Subject:
Biological & Biomedical Sciences
Type:
Lab Report
Language:
English (U.S.)
Document:
MS Word
Date:
Total cost:
$ 17.28
Topic:

Public Health Report

Lab Report Instructions:

For section 1— you need to state the case, list the hypothesis you came up with, note
some of the things you wanted to know, state additional history, what the doctor’s
diagnosis was and finally what the public health folks did. You did all of this day one.
For sections 2 - you will show the results — you did not have to do this part but you got
results from the health department — the results are in a document entitled Public Health
report.
You need to state the methods you used to do the enumeration and use the different
media. You should have a table that has the results of the enumeration and the growth on
the different medias. You should also state what the Gram stain should be. That is Gram
reaction and shape. The last column should state – if you can – what each organism
isolated is from the data you have.
Table 1 - findings for the CFU/ml for each organism, then the results for the three
different differential/selective medias. Then a column with the Gram stain results and
finally a column with the final ID. Since I am giving you the organisms ID in the next
section the names and the results on the different media should allow you to come up
with the Gram stain results. If you want you could have a figure with borrowed Gram
stains of these organisms.
Table 2 - The IDs with the source —- anything else you think would be could in this
table.
Here is the final ID of the organisms (Growth on A & B Salmonella typhi, C E. coli, S.
aureus, D S. aureus, and E. Pseudomonas). You need to discuss what you think of your
findings. That is are you surprised to find the organisms you found in the places you
found them? Are they dangerous?
4. Finally you will end with a discussion of food borne infection – and the public health
implications and the patient

Lab Report Sample Content Preview:

Public Health
Student’s Name
Institutional Affiliation
Public Health
The process of laboratory identification of bacteria could be challenging and interesting as well as it requires one to identify the clues. The public health officials are investigating a situation where 60-years-old women suffered loose watery stools 8 to 10 times a day and severe abdominal cramps and headache for three days. On the fourth day, the patient also observed blood in stool leading to the presumption that the primary diagnosis was food poisoning. The public health officials were, therefore, interested in determining the cause of the gastrointestinal problems experienced by the geriatric woman. The patient has no history of food intolerance, gastrointestinal disorders, or stomach sensitivities. The client eats with family at home but before the onset of the symptoms, she had a meal at a nearby restaurant with the family. The family ate similar food but the woman also ate burger which is suspected to be the cause of the clinical features. The doctor’s primary diagnosis was food poisoning and, therefore, sent stool samples to the laboratory for analysis and evaluation. Public health officials visited the restaurant and sampled all food eaten by the woman and sent to the laboratory for evaluation and analysis.
Materials and Methods
A glucose fermentation test was performed to test the ability of the bacteria to utilize glucose as an energy source. The tube used for fermentation contained phenyl red, a broth with specific sugar and dye. The isolated gram negative bacteria were then moved to the inoculating loop into the broth where the tube was incubated at 370C and the findings recorded. Simmons’ Citrate is a test that was used to determine if gram negative bacteria produced citrase. The enzyme breaks down citrate to produce carbon as a source of energy. The process alkalinizes the agar changing its color to blue. If the color changes to blue for Simmon’s slant then the bacteria are positive for citrase, otherwise the test is negative if there is no visible color change. The gram negative bacteria that were isolated were transferred into an inoculating loop and a colored test tube surface. The test tube was then inoculated at 370C and the findings recorded. Nitrate reduction test was also done to determine if the bacteria produced nitrite or nitrate reductase.
Findings
Biochemical Tests
Carbohydrates

14

Starch (+ve)

Few drops of iodine added. Blue-black color. Indicator for the presence of starch. Negative (-ve) for Amylase.

15

Phenol Red Glucose (+ve)

Turns from red to yellow color. Gas seen at the top in the Durham tubes. Positive (+ve) for glucose use, positive (+ve) for dextrase enzyme, positive (+ve) for gas.

16

Phenol Red Lactose (+ve)

Yellow color, Gas seen at the top in the Durham tubes.Positive (+ve) for lactose use. Positive (+ve) for dextrase enzyme.

17

Phenol Red Sucrose (-ve)

Red color. Gas bubbles seen at the top in the Durham tubes. Negative (-ve) for enzyme dextrase.

18

Methyl Red (MR)
(+ve)

Red color, no neutral color seen. Used to identify enteric bacteria based on h...
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