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Pages:
1 page/≈275 words
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1 Source
Style:
APA
Subject:
Life Sciences
Type:
Coursework
Language:
English (U.S.)
Document:
MS Word
Date:
Total cost:
$ 4.75
Topic:

PCR and the Enzyme Used for This Process

Coursework Instructions:

PCR has been developed as a technology for correcting gene defects, thereby advancing the diagnostic aspects of molecular biology, especially for infectious and diagnostic diseases.
What is PCR? Describe the technique in detail.
What enzyme is used in this process and how is the enzyme used in PCR different from others?
What is that enzyme's function in the cell?
How has PCR changed microbiology?

Coursework Sample Content Preview:

PCR as a Technology of Correcting Gene Defects
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PCR as a Technology for Correcting Gene Defects
Detailed Description of PCR
PCR is a laboratory technique for replicating a specific DNA sequence. The method can generate millions of copies of the target DNA segment from the original DNA molecule (National Cancer Institute, n.d.). The original molecule only contains a small amount of that DNA. The cyclic nature that allows for the replication of DNA sequences in a series of 30 to 35 cycles allows for producing many copies quickly. Each cycle involves three steps: extension, annealing, and denaturation.
The Enzyme Used in the Process, Why It is Unique, and Its Function in the Cell.
Taq polymerase is the DNA polymerase used as the key enzyme in PCR reactions. It utilizes existing DNA strands as templates for generating new strands of DNA. A unique aspect of this enzyme that makes it different from others is its...
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