Proposed Intervention and Expected Outcomes

Proposed Intervention and Expected Outcomes
Student Name
Institutional Affiliation
Proposed Intervention and Expected Outcomes
Proposed Intervention
The intervention is the adoption of evidence-based practice—the adoption of next-generation sequencing to address the antimicrobial resistance menace in society. The next generation will include incorporating the k-mers in the establishment of the genes responsible for antimicrobial resistance. The method is currently the saving grace for the world, given the rampant antimicrobial resistance in many parts of the world. The intervention is appropriate and realistic for the selected practice setting since it increases microbial data availability and continually reducing costs, making sequencing a feasible antimicrobial resistance surveillance instrument. Organizing sequencing information is a critical pre-processing phase before antimicrobial resistance gene evaluation. Illumina-generated short reads could either be processed using assembly-centered techniques, where sequencing reads are categorized into contigs (contiguous fragments) first and then annotated by comparing with public or custom reference databases; or directly evaluated using read-centered techniques, where resistance factors are forecasted by charting reads to a reference database directly (Boolchandani et al., 2019).
Expected Outcomes
It is expected that k-mers’ testing of the antimicrobial resistance genes and single-nucleotide polymorphism in S. aureus and M. tuberculosis would reveal that either S. aureus or M. tuberculosis has a high sensitivity hence responsible for the antimicrobial resistance to antibiotics. The expected outcome relates to the project objectives since it would show whether the use of next-generation sequencing reduces antimicrobial resistance (Balkhaira et al., 2019). In that vein, the outcome would confirm the efficacy of next-generation sequencing. That said, a reduction of 52% will be a manifestation of the efficacy of next-generation sequencing in fighting antimicrobial resistance. The outcomes coincide with the PICOT question. The PICOT question is: Within a community with high cancer patients’ prevalence, how effective will the introduction of next-generation sequencing be to eliminate antimicrobial resistance compared to the use of common antimicrobial drugs in the entire period of antimicrobial resistance?
Method to Achieve Outcomes
The steps of next-generation sequencing include library preparation, cluster generation, sequencing, alignment, and data analysis. The next-generation sequencing library is created at the library preparation step by fragmenting the gDNA sample and ligating particular adapters to both the fragment ends. The library is loaded into the flow cell in cluster amplification, and the fragment is crossbred to the stream cell surface. The respective destined portions are augmented into a clonal group via bridge amplification. In the sequencing phase, reagents, including fluorescently marked nucleotides, are included, and the initial base ...