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Health, Medicine, Nursing
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English (U.S.)
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Topic:
Polymerase Chain Reaction and Microbiology
Essay Instructions:
PCR has been developed as a technology for correcting gene defects, thereby advancing the diagnostic aspects of molecular biology, especially for infectious and diagnostic diseases. Research on PCR. Based on your research, respond to the following:
What is PCR? Describe the technique in detail.
What enzyme is used in this process and how is the enzyme used in PCR different from others?
What is that enzyme's function in the cell?
How has PCR changed microbiology?
Please use the APA guidelines to include in text citations
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Essay Sample Content Preview:
Polymerase Chain Reaction and Microbiology
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PCR
PCR is a reaction used to magnify small DNA portions. Genetic and molecular analyses of DNA require a significant amount of its samples, and PCR amplification facilitates the study of isolated DNA pieces. DNA produced after PCR magnification is used in laboratory functions such as Human Genome Project (HGP) mapping techniques, DNA fingerprinting, and genetic disorder detections (National Human Genome Research Institute, 2015). PCR process involves heating the DNA samples for it to separate into two elements of single-stranded DNA.
PCR , its Enzyme and the Function
The enzyme used in the PCR process is referred to as Taq polymerase. The enzyme is used PCR due to its high fidelity in DNA synthesis (Imanaka, 2011). After the DNA is heated to separate into two pieces, the enzyme builds two new strings of DNA using the parent string as the master. The process leads to duplication of original DNA with the newly formed molecules contains each strand of the old and new DNA. The freshly created strands develop other new copies through a continuous process, resulting in more than one billion original DNA duplicates (NHGRI, 2015)....
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