Hanging-Drop and Wet-Mount Preparations and Simple and Gram Stains

Microbiology
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Microbiology
Exercise 1: Hanging-drop and wet-mount Preparations
In true mobility, there is a change in position regarding the original point. For instance, cells can flow autonomously and in a specified orientation. However, a Brownian movement is vibratory, which occurs at constant rates around a specific position.
In bacterial mobility, the morphological structure responsible for the movement is flagella. Possessing flagella on the exteriors of cells serves as a molecular drive or screw that generate thrust. Flagellum is a hairlike mechanism acting primarily as an organelle for cell movement.
Discarding wet preparation in biohazard containers is done to eliminate infections. That is necessary for laboratory safety as wet mount has living microorganisms. A small amount of bacteria suspension placed on the slide also help to produce clear specimen images under a viewing microscope.
Hanging drop is a complex method or procedure that allows long-term observations and a more vivid viewing of mobility. The technique is often carried out without the addition of stain. With a hanging drop, the organism cannot dry out rapidly and could live longer. On the other hand, a wet-mount preparation is valuable in helping to see living specimens and show the quality state of being motile, thereby helping in identifications.
Exercise 2: Simple Stains
A basic dye in microbiology is a positively charged stain inside a cell, while an acidic dye is negatively charged, affecting outside a cell. Bacterial surfaces repel acidic dyes, stain the background and leave a bacterial sell clear (Corrales et al., 2020). Basic dyes bind directly to stain negatively charged surfaces.
A slide that needs to be stained gets fixed to prevent washing off during staining. That can get done by passing a slide through a flame. Another alternative of fixation is flooding the slide with methanol, as this prevents liquid specimens from being washed off. The ...