Impact of Nanofabrication in the Field of Biology

Relationship Between Fibroblast Associated Tumor Environment and Vascular Structure Networked Tumoroid
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Relationship Between Fibroblast Associated Tumor Environment and Vascular Structure Networked Tumoroid
Introduction
The contribution of nanotechnology in the field of biology is an important aspect, especially in the study of cell structure and function. Nanotechnology has enabled the formulation of new ways of measuring and detecting biology in both in vivo and in vitro. The fact that the nanoscale devices have the ability to sense a minute difference in the level of single molecules and the single cells has elevated the world of discovery in studying most cells and finding solutions to major problems regarding cells (Wang & Pumera, 2015). According to research, the in vitro 3D tumor models have the same characterization as the in vivo cancer tissues. The in vitro cancer replica have enabled not only the use of basic cancer research but also the application of anticancer drug innovation. The application of the 2D tumor models has been in use for decades though its effectiveness is becoming void since the invention of the 3D tumor model. 2D is still in use in cancer therapy, anticancer drug development, screening drugs, and other research related to cancer. The advantage of the 2D is that it lacks the complexity of 3D cells to the extracellular matrix and the cell-to-cell fretwork of cancer. However, the application of the 3D model has proven a lot of significance in the cancer treatment sector. According to research, cancer tissues that are characterized by ECM and stomatal cells can create a tumor microenvironment, which is characterized by an active cell to cell interaction and cell to ECM interaction (Wang & Pumera, 2015). In this interaction, there is an exchange of various factors not limited to biophysical and biomedical contributing to cell resistance to cancer drugs. In this research, there is a description of the 3D in vitro lung cancer model that is cultured in a microfluid channel, thus providing a cohesive interaction of the TME. However, in as much there is an advanced development in finding ways of developing anticancer drug development, other new strategies of improving in vivo and in vitro are still on its viable, especially with the recent development of 3D multicellular tumor spheroids, which has the same characteristics as tumor tissues thus duplicating the in vivo TME.
Hypothesis
The aims of the experiment are to;
* To find out the structures formed in the microfluidic channel
* The impact of the in vitro tumoroid formation
Aim 1
To achieve this aim, the experiment is performed after the formation of the tumoroid. It involves stretching a sheet of HUVEC between the tumoroids and left for a period of 4 to 6 days. The expected results of this experiment are the development of vessel-like tubular anatomy after 4 to 6 days stretching of the tumoroids that connect the micro tumors after seeding of the cells for ten days (Amemiya et al. 2021). In addition, the vessel ought to contain the collagen IV matrix and HUVEC, thus indicating the endothelial markers connected to micro tumors, which is similar to blo...