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Experimental Protocols and Techniques Life Sciences Research Paper

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3. Experimental Protocols and Techniques Due: Friday of Week 5 at 5:30 PM CDT (Oct. 30) Class Lectures: Ch. 9. The Gaseous State (Week 4) THE CHEMISTRY (with the lectures) Read the paper entitled “UV Dosage Levels in Summer: Increased Risk of Ozone Loss from Convectively Injected Water Vapor,” located at https://science.sciencemag.org/content/337/6096/835/tab-pdf, and think critically about the chemistry applied in the experiment. Specifically, you should write about the following: 1. Review ideal gas laws. Identify applications of the ideal gas law in the project paper and provide a short comment. Try to identify examples where the authors exploit an ideal from the ideal gas law but do not explicitly invoke it. 2. Use your own words to explain how the concepts involved with the ideal gas law are used or are necessary to enable the experiments or chemical understanding in the paper. PROTOCOLS AND TECHNIQUES The experimental (methods) section is an extremely important part of a good science paper, as this is the place to communicate with readers on how to reproduce the experimental work. To perform experiments safely and productively, you need both well-documented experiment protocols and good lab techniques. A protocol should allow you to work systematically during an experiment, to minimize potential mistakes. For this week’s lab report, review the project paper, and identify the protocols and techniques used in the experiment. You may create a flow chart for the protocol and present a list of instructions (including equipment) used for the experiment. This may include but is not limited to, the experimental setup, experiment length, preparation, working conditions, data collection, and analysis. All important parts of the experimental procedure must be included. That being said, many wellknown techniques and manipulations are implied and so can be referenced without pedantic details. (You can recognize well-known techniques even if they aren’t well known to you. Authors will often cite other groups regarding such techniques, but when they cite their own papers, it may be somewhat specialized. Also, techniques mentioned with minimal explanation are likely well-known techniques, but a quick literature search on google scholar can probably help you to identify them and also to properly cite them.) EXPERIMENTAL FINDINGS After following the experimental protocols and applying good lab techniques, presumably, you will have obtained satisfactory experimental data. It is now time to perform analysis that will, hopefully, yield a clear and novel conclusion. Explain in your own words the analysis strategy and choices/compromises made by Anderson and coworkers. TABLES AND FIGURES Data tables and figures may be used to present the experimental results. Figures are graphical depictions that can tell a story at a glance and should be simple and without extraneous text. Every figure must be numbered and include a caption that describes its contents. Captions are normally phrases and contain a period in the end. More information on creating good figures can be found in The ACS Style Guide. Examine the figures from the current project paper, provide comments, and conclusion(s) from these presentations. Specifically, look for a detail not explained in the paper and hypothesize what might be causing it. For your report, please include one annotated figure and a caption of your own creating explaining the figure/annotation. You may assume that the reader is familiar with the Anderson paper. Any figures that you include in your lab report should either be original (that is, made by you) or adapted from a cited source and annotated by you. Please do not simply include a figure from a paper without your somehow adding something meaningful to it. Lab Report this week: Your report should include all three components discussed above, i.e., the chemistry discussion related to the class lectures, experimental protocols and techniques, and the figure analysis and experimental findings. Once you have gathered all the data with your writing, submit your report to the Canvas assignment page. Your report will be about four pages long including one page explicating applications of the ideal gas law, one page containing your flow chart and protocol (it’s ok for this to be longer than 1 page if that helps keep the charge legible for the TAs), one page on the data analysis and strategy, and one page containing an annotated figure and caption. GRADING Section Possible Points Points Earned Ideal Gas law examples and discussion 30 Protocols and techniques 20 Experimental analysis strategy discussion 20 Figure 20 Writing style and professional presentation 10 TOTAL 100

Research Paper Sample Content Preview:

Experimental Protocols and Techniques
Name Course Instructor Date
1. Ideal Gas law examples and discussion
In the ideal gas law, the pressure and volume of a gas are equal to the ideal gas constant, the temperature, and the amount of the substance .in other words, the pressure, volume, temperature, molecular weight, and amount of the gas are linked each gas has its own weight and pressure decreases with altitude. In the experiment, ozone loss is depicted, beginning with the inorganic form of chlorine. There is a reaction of chlorine nitrate (ClONO2) and hydrochloric acid (HCl), and under the ideal conditions of temperature, the free-radical forms are formed. 
The research by Anderson et al. and colleagues highlights that wet conditions and low temperatures influence the heterogeneous reactions linked to chlorine monoxide (ClO) in the Arctic lower stratosphere. Chlorine activation depends on the presence of water vapor, and the temperatures, when water vapor injected into the stratosphere, alters the free-radical chemistry of chlorine and bromine. There are changes in the lowers stratosphere during summer where the water vapor temperature and the pressure are considered to understand how this affects chlorine activation. Both the high water vapor concentration levels and low temperatures influence the conversion and collection of the inorganic chlorine to the free-radical forms.
Bromine and chlorine reactions affect the lower stratosphere and cause ozone depletion. Chlorine that is part of the (Chlorofluorocarbons) and Beaks up when there is contact with sunlight. Chlorine monoxide (ClO) is the result of chlorine reacting with ozone (O3), and when CIO meets with oxygen molecules, there is the release of chlorine (Cl) and oxygen there is a catalytic cycle of chlorine. In the ozone destruction cycle, where there is chlorine, sunlight is required in the form of solar ultraviolet radiation to facilitate the cycle where different forms of chlorine are formed and reformed.
2. Protocols and techniques
Temperature
Water vapor mixing ratioHeterogeneous Conversionof Inorganic Chlorine
Requires
Aerosol reactive surface area
Injected water vapor
Pressure (mb)Temperature (K) (mb) H2O (ppmv)
18 ppmv12 ppmv5 ppmv
In situ observations of the injected water vapor (H2O) from NASA aircrafts is determined from flights where there are observations in outflow the US during summer. There is the observation of water vapor between 5 and 20 H2O (ppmv) and pressure (mb) at between 20 and 120 mb NOy , Cl y , Br y , and HOx evolved when there are photochemical reactions and results are the species, which are different mixing ratios of HCl, ClONO2 ,Cl2 , NO2 , NO, ClOOCl, and ClO” (1).
To determine how photochemical catalytic free-radical chemistry affects ozone depletion and the lower stratosphere there was observation of the conditions under unperturbed conditions where H20 has not been injected into the atmosphere (1). At 5, 12 and 18 ppmv water, there is depiction of the relationship between the original concentration level and the ozone for up to 80 hours.
This was followed by determining how convectively injected water vapor affects the stratosphere in the northern hemisphere (1). The mid-latitude cond...
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