1 page/≈275 words
Health, Medicine, Nursing
Acute Promyelocytic Leukemia: Molecular Diagnostic Procedures (Essay Sample)
open Number of sources 5 and more from 2006 to 2010 figurs is important to show the result I need the procedures and the results please make it interesting source..
ACUTE PROMYELOCYTIC LEUKEMIA: MOLECULAR DIAGNOSTIC PROCEDURES
(02, September, 2010)
Acute Promyelocytic Leukemia: Molecular Diagnostic Procedures
Raymond, et al, (2008) and Massey, et al. (2006) says that on Cell Surface-Antigen Immunophenotyping, the separation of borne marrow mononuclear cells was by Ficoll-Hypaque density sedimentation and analysis of peripheral-blood was by a whole blood with the Q-prep system. By the use of dual-color fluorescence of the cells gating depending on size and the forward-scatter analyzed on the profile flow cytometer of EPICS while the expression of a panel of the antigens, including CD33 and CD16. All sample cells were analyzed up to three gate clusters.
In the method of Molecular Cytogenetics (Fish), part of material fixed in carnoy to make fish slides carried out in accordance with the probe supplier`s instructions. Slides analysis was by fluorescent microscope with rhodamine, DAPI, FITC and the triple band pass filters. The best images were taken by the CCD camera as shown if figure 3. Slide with having above 50% cell with fluorescent dots were selected and analyzed by two observers. There were two types of count; normal count, without rearrangement and present rearrangement. Cases with above 10% were looked at as positive. (Ghaffari, 2006)
With the method of PCR, quantitative Real-time PCR, the extracted RNA were subjected to 2 different quantitative real-time reverses transcription-polymarase chain reaction methods to detect the two types of PML/RAR-α fusion transcripts abl amplification is done as the control and reference for relative quantification. If PML/RARA (15:17) translocation is shown then the result is positive if the ratio is 0 then the result is negative. (JÃ¼rgen et a...
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