Moist, Dry Heat, and the Autoclave
Week 5 – Review Sheet
Exercise 1: Moist and dry heat
1. Howaremicroorganismsdestroyedbymoistheat?Bydryheat?
2. Are some microorganisms more resistant to heat than others? Why?
3. Ismoistheatmoreeffectivethandryheat?Why?
4. Whydoesdryheatrequirehighertemperaturesforlongertimeperiodstosterilizethandoesmoist
heat?
5. What is the relationship of time to temperature in heat sterilization? Explain.
Exercise 2: The autoclave
1. Define the principles of sterilization with an autoclave and with a dry heat oven.
2. What pressure, temperature, and time are used in routine autoclaving?
3. What factors determine the time period necessary for steam-pressure sterilization? Dry- heat oven sterilization?
4. Why is it necessary to use bacteriologic controls to monitor heat- sterilization techniques?
5. When running an endospore control of autoclaving technique, why is one endospore preparation incubated without heating?
Please use the APA guidelines to include in text citations
Please make sure!! General note on TII scores: When you submit your worksheet assignment, it is automatically evaluated by Turnitin for originality. The TII score must be below a 49% Thank you
Moist, Dry Heat, and The Autoclave
Student’s Name
Institutional Affiliation
Exercise 1: Moist and Dry Heat
Question 1: Moist heat eradicates microorganisms by coagulation and distortion of enzymes and proteins present in the organisms. Research indicates that moisture impacts proteins’ coagulation temperature and the temperature at which the microorganisms are destroyed. On the flip side, dry heat eliminates microorganisms by causing protein clotting.
Question 2: Yes, some microorganisms are more resistant to others. Microorganisms whose proteins have plenty of disulfides and covalent bonds between sulfur atoms have a high possibility and stability of heat resistance; thermophilic bacteria may thrive in extreme heat.
Question 3: Moist heat has a better performance than dry heat since it penetrates deep into the tissues at constant temperatures. Additionally, moist heat can potentially adjust tissue temperatures and achieve a more vigorous temperature receptor feedback. Moist heat patients also report a considerable relief of symptoms (Polar Products, 2021). The increased blood circulation in the target region by moist heat also increases the recovery process of the patients. Enhanced circulation help in the production of fresh blood and eliminates waste products that can hinder the healing process.
Question 4: Dry heat requires more sterilization time than moist heat since it does not penetrate well. Dry heat has poor heat penetration, resulting in prolonged exposure and high temperatures (Sastri, 2016). Also, dry heat is unsuitable for plastics since they have low thermal transmission characteristics and hardship in ensuring that the entire product is open to get the temperature required for a stipulated duration.
Question 5: Sterilization usually takes less time with high temperatures and a longer time when the temperatures are low. Precise temperatures must be maintained to ensure microbicide’s actions. A temperature of 121°C and 132°C are the two ordinary temperatures for steam sterilizing. To eliminate microorganisms, these two temperatures and another high of 830 must be maintained for a short time.
Exercise 2: The Autoclave
Question 1: Autoclave operates under the principle of moist heat specializa...
👀 Other Visitors are Viewing These APA Essay Samples:
- 503 Mod 4 Case: Family-Building in the Artificial Reproductive Technology (Art) Era2 pages/≈550 words | 2 Sources | APA | Health, Medicine, Nursing | Essay |
- Student Success Health Science2 pages/≈550 words | No Sources | APA | Health, Medicine, Nursing | Essay |
- Francimar Nipo Bezzerra; Telma Marques da Silva; Vania Pinheiro Ramos4 pages/≈1100 words | 2 Sources | APA | Health, Medicine, Nursing | Essay |
- Population Health Inputs and Their Effects4 pages/≈1100 words | 10 Sources | APA | Health, Medicine, Nursing | Essay |
- 2004 Indian Ocean Earthquake And Tsunami2 pages/≈550 words | 3 Sources | APA | Health, Medicine, Nursing | Essay |
- Week 2: Finding Sources of Evidence. Health, Medicine, Nursing Essay1 page/≈275 words | 3 Sources | APA | Health, Medicine, Nursing | Essay |
- Effective Interagency working in Healthcare Health, Medicine Essay7 pages/≈1925 words | 35 Sources | APA | Health, Medicine, Nursing | Essay |