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Topic:

Control Cultures, Mixed Cultures, and Pure Cultures in Evaluating Disinfectants

Essay Instructions:

Week 6 – Review Sheet
Exercise 1 - Disinfectants
1. What does bactericidal mean? Bacteriostatic? Virucidal? Fungistatic?
2. Why are control cultures necessary in evaluating disinfectants?
3. What factors can influence the activity of a disinfectant?
4. Why do microorganisms differ in their response to disinfectants?
5. What microorganisms are most susceptible to disinfectants?
Exercise 2 - Antimicrobial Agent Susceptibility Testing and Resistance
1. What is meant by antimicrobial resistance? Susceptibility?
2. Why are pure cultures used for antimicrobial susceptibility testing?
3. Would it be acceptable to use a mixed culture for this test? Why?
4. List three factors that can influence the accuracy of the test.
5. When performing a broth dilution test, why is it necessary to include a growth control tube? A sterility
control tube?
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Essay Sample Content Preview:

Week 6 – Review Sheet
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Week 6 – Review Sheet
Exercise 1 - Disinfectants1. What does bactericidal mean? Bacteriostatic? Virucidal? Fungistatic?
* Bactericidal is an agent capable of targeting and killing bacteria, and some bacteria have resistance to certain bactericides (Tighe & Brown, 2019).
* Bacteriostatic is an agent capable of preventing the growth of bacteria.
* Virucida lis a virus-destroying agent.
* Fungistaticis an agent that inhibits the growth of fungi.
2. Why are control cultures necessary in evaluating disinfectants?
* Control cultures are necessary in evaluating disinfectants because they help determine whether the disinfectant killed the bacteria or not.
3. What factors can influence the activity of a disinfectant?
* The factors that can influence the activity of a disinfectantinclude the pH levels, temperature and time of exposure, disinfectant concentration, and the number of microorganisms.
4. Why do microorganisms differ in their response to disinfectants?
* The type of microbes present, their structure, and their resistance to disinfectants influence how microorganisms respond to disinfectants.
5. What microorganisms are most susceptible to disinfectants?
* Microorganisms most susceptible to disinfectants are gram-positive bacteria, viruses, and hyphae.
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