Useful Antibiotics, Production of New Metabolite, and the Jumping Genes

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Question 1: Most medically useful antibiotics interfere with either peptidoglycan synthesis or ribosome function. Why would the cytoplasmic membrane be a poor target for antibacterial medications?
The antibiotics ought to depict selective toxicity, where they can selectively destroy the bacteria system while not harming the host. Most antibacterial medications do not have a selective bacterial cytoplasmic membrane-targeting mechanism. Thus, such medications that are targeted at bacterial cytoplasmic membrane can equally damage cells in human systems, including the nervous system and kidneys. However, some antimicrobial agents directly target a part of bacterial cytoplasmic membranes in both Gram-negative and Gram-positive bacteria. These cytoplasmic targeting antibacterial agents are mainly cyclic peptides which have a rigid binding site capable of binding a lipid component. The cyclic peptides have binding sites that target bacteria, rather than being toxic to host cells (Epand et al., 2016).
Question 2: You are a microbiologist working for a pharmaceutical company and discover a new metabolite that serves as medication. You must oversee its production. What are some of the factors you must consider if you have to grow 5,000-liter cultures of bacteria?
During the process of the introduction of new antibacterial drugs, it is important to test its effectiveness by subjecting it to sensitivity and susceptibility tests. The cultured bacteria are exposed to such a new drug, which is the new metabolite in this case. A critical action entails a step is to grow different bacteria through a culture that will be used to examine the effectiveness of the new metabolite. The culture media is necessary for microbiological tests. However, it is essential to grow and obtain pure, uncontaminated cultures through proper growth, cultivation, and selection of microorganisms. Thus, it is recommended to be aware of factors or variables that interfere with bacterial culture growth. First, the quality and type of media affect the final culture outcomes. In the absence of quality culture media, the probability of getting accurate, predictable, reproducible, and repeatable tests becomes reduced. A microbiological culture medium represents a substance that facilitates the growth, support, and survival of bacteria. Culture media, among other things, should contain nutrients, growth-promoting factors, energy sources, buffer salts, minerals, metals, and gelling agents. Gelling agents are needed for solid medium.
There are typically two forms of culture media, which include liquid cultures, often called broth, and solid and semi-solid cultures, ordinarily called agar. There are further subtypes of the two media, for instance, growth media, which allows the growth of heterotrophic microorganisms, transport culture media that preserve such organism, enrichment media that works by increasing the cultured organism numbers, and selective growth culture media. The selective media will allow the growth of specific bacteria and restrict the growth of others. The pharmaceutical microbiology laboratory, like the one to test the efficiency of metabolite...