Identifying and Isolating Microbes from the Environment (Lab Report Sample)
IF YOU HAVE QUESTIONS IMMEDIATELY MESSAGE ME!!!
IT IS A REPORT STRICTLY FOLLOW THE FORMAT
this paper has to be writteN in in a research from. it must have am abstract, introduction, methods, results, and discussion.
i will be attaching a copy of the school lab's manual so you can read the protocol of the methods and be able to write them in sentences just like any scientific report please. also i will be attaching the figuires and the graphs that you will have to use in order to write down the results section well.
The introduction: it has to be from broad to specific about microbes (there will be a complete attached form of the protocol and a background about the experiment, a hypothesis is not required at all)
Methods: has to be in past form completely with sub-titles. Make sure to write them in sentences and use the numbers as they are written in the protocol. (DONT COPY the protocol because it is written as a recipe cook book. it has to be in complete sentences in the past form while using all of the values mentioned in the protocol (numbers, name of solutions..etc)
Results: you will have to put all of the tables and images i will post as well then write a fig. and a brief 4 sentences about that fig so that the reader may know what does this fig talks about. Then you have interprent the raw data from the figs in the results section. (just like any scientific report)
Discussion: here you gonna talk about in deep about the results and you will mention why does some of the sequences didnt work. in addition, compare between the microbes we sequenced..etc
i will further explain once i post all of the attachments
you will use 6 sources and they are only gonna be on the introduction and discussion only.
I expect all of those sequences to be BLASTED and included in the results and expanded upon in the discussion, along with the image of the gel, a written summary of the table in excel, where you talk about the diversity in the colonies found in each built environment, as well as a table with the DNA concentrations and 260/280 ratio (as these can be used to discuss why your sequencing for some of the samples may not have worked in the discussion section). You should also include what organism(s) were found where, but save the explanation of those organisms for the discussion
i have already sequenced and blasted the organisms and they are attached on a word file for you. out of 8 only 3 we were able to blast and know what microbe they are.
please dont forget the ciatation page to be alone and you may use both Primary and Secondary sources
the table colony data (sheet- section 15) you will not put the table on the paper, you have to only interpret the data on the results sections in deep manners.
in all of the excel files, Use only -SECTION 15- Data not section 12
Identifying and isolating microbes from the environment
Microbes are found in different locations and surface types, and researchers have in the past relied on culturing microbes to isolate and identify the microbes. Increasingly, DNA sequencing has been utilized as a more effective way to identify microbial characteristics in the built environment different samples were collected in the biology lab and the hallway where gram staining and 16S rRNA sequencing was conducted to identify the various microbes on the surfaces. After analysis, Micrococcus luteus strain, uncultured bacterium clone and staphylococcus sp strains were identified, but the other five could not be identified. Sequential analysis was possible through the use of FINCH and BLAST programs, and gel image was also utilized for analysis. Human contact was identified as a strong predictor of microbes form the built environment, while spatial proximity, the location and the time the study was conducted could also explain microbial diversity.
Microorganisms are everywhere in the built environment and culturing the microbes is necessary to identify the microbes present in this environment. Even as this approach has been used over time there is a drawback since some microbes cannot be cultured. DNA sequencing is equally cheap and effective since it is possible to identify the microbes when here are no cultures. As human have created the built environment the microhabitats have become more diverse than ever before, and microorganisms colonize these habitats (Kelley & Gilbert 1). When compared to the natural world, the microbial life of various microorganisms is remarkably different as there are more people in moving in and out of the built environment. The source of the microbes may be human, water and environmental elements, while even insects and animals can transport the microbes.
Even as culture based methods are used to identify and isolate microbes, they underestimate the diversity of microbial communities. The pattern of occupancy influence bacterial communities in the built environment (Stephens et al 1). However, in the case of buildings with moisture problems, the fungal communities thrive even when they are from the outdoor environments, while humans also influence their presence (Stephens et al 1). Additionally, the building characteristics like surface materials, design and ventilation strategies affect the diversity of microbial communities (Meadow et al 2).
In past research the culture based studies have shown that microbes are everywhere in the built environment, but we still do not fully understand microbial diversity (Kelley & Gilbert 1). Isolating and identifying the microbes from the build environment is necessary to understand how the building attributes and biodiversity influence human health. Studies on microbes in the built environment have mostly focused on identifying pathogenic strains, bioaerosol concentrations and the presence of culturable strains (Kembel et al 1469-1470). More research to understand the microbial diversity of the built environment is necessary. This study applies the culture the gram stain and 16S ribosomal sequencing approaches to identify and isolate microbes from the built environment.
Setting and study design
Different swab samples were taken from different chairs in the biology lab and benches in the hallway on the 3rd floor. Subsequently, the microbes were cultured from the swabs where the species richness was identified to determine microbial diversity. All the samples taken were labeled depending on their source location, and this was necessary to provide a detailed description of the type of microbes depending on their location and the built environment.
Gram staining procedure
The samples ...
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