How do substrate concentration and pH affect enzyme-controlled reaction

Lab report: how do substrate concentration and ph affect enzyme-controlled reaction
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Introduction
Enzymes are organic catalysts that speed up a reaction and produce an ordered, stable reaction system. Products of the reaction are formed depending on the requirement of the experiment. Each specific enzyme controls each reaction in a series of metabolic reactions.
Sequence of enzyme-controlled reactions is as follows;
Substrate+ enzyme=enzyme-substrate complex=enzyme+ products
Background information:
Reactions are important and necessary in various chemical and biological processes. Enzymes are used to catalyze reactions, hence reducing the time taken to complete a reaction. Enzymes were first discovered in the 18th and 19th century in the manufacture of beer. Other enzymes were later discovered during biological tests in the study of human physiological processes. Enzyme amylase is one of the enzymes facilitates the human digestion in the human gut.
Objectives:
The purpose of the experiment is to determine the effects of substrate concentration and pH on the initial rate of an enzyme-catalyzed reaction. This will be done by virtual simulation. Each test tube used for simulation contains the same amount of enzyme solution. The pH levels are adjusted, or some amount of the substrate is added to collect the necessary data.
Number of Molecules of Product Formed per Minute (x106)Amount of Substrate (g)pH 7pH 3pH 11Test Tube 10.5721924Test Tube 21.01453949Test Tube 32.030082103Test Tube 44.035096121Test Tube 58.035096121Table  SEQ Table \* ARABIC 1: Table of results with change in the substrate concentration


Table  SEQ Table \* ARABIC 2: Fig 1: graph of the substrate concentration
Number of Molecules of Product Formed per Minute (x106)pH0.5 g2.0 g8.0 gTest Tube 13198296Test Tube 2539168198Test Tube 3772300350Tes...