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Essay Available:
Pages:
11 pages/≈3025 words
Sources:
15 Sources
Level:
APA
Subject:
Life Sciences
Type:
Lab Report
Language:
English (U.K.)
Document:
MS Word
Date:
Total cost:
$ 49.01
Topic:

Activation studies and isolation of microbes (Lab Report Sample)

Instructions:

Topic: Activate secondary metabolisms in Streptomyces spp. and isolate microbes from terrestrial soil samples. The order should include these subheadings: 1- Abstract: The purpose of this study was activated secondary metabolisms in Streptomyces spp, by using natural chemical cues (LPS, mycolic acid) and biological cues (Tuberculosis cells{TB}).Also, I isolated microbes from 4 terrestrial soil samples during the incubation time for microbes( Streptomyces spp). 500 words. 2- Hypothesis: Streptomyces spp contain secondary metabolite genes that are not transcriptionally activated under standard cultivation conditions, but that can be activated by natural chemical cues (e.g. LPS, mycolic acid)and biological cues (Tuberculosis cells). 150 words. 3- Results: I will upload the results in a file. 4- Discussion: Also,I will upload it in a file. 5- Conclusion. Also, I will upload diagrams and examples that you can use. So, more details about the report (order) will be uploaded later. I uploaded the files. The first one regards to my order while the other will help you.I included the review and the method to my report to see the organisation of it. In the same file I also include the other parts regard to my order ( abstract,hypothesis,results, discussion,limitation and conclusion . USE this as well - https://onedrive.live.com/view.aspx?resid=A043E1BF818B4839!120&cid=a043e1bf818b4839&ithint=file%2c.pdf&app=WordPdf&authkey=!AABobZMZdx6EVOQ Thanks

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Content:
Abstract
Bioactive compounds derived from the natural world are responsible for many of the therapeutic effects that our ancestors experienced, and at present, these compounds serve as the framework by which scientists develop novel drugs. Streptomyces is known as the largest genus of Actinobacteria, and this genus belongs to the family Streptomycetaceae. Streptomycetes, just like other Actinobacteria have genomes with large amount of GC content and they are Gram-positive. They are typified by a compound secondary metabolism, and they generate more than â…“ of the clinically valuable antibiotics of natural origin such as chloramphenicol and neomycin. The purpose of this study was to activate secondary metabolites inStreptomycens spp. In normal laboratory conditions, the microbes would not produce secondary metabolites. Therefore, chemical activators (Lipopolysaccharideandmycolic acid) and biological activator (Tuberculosis cells or Tb) were used in this study to activate silent secondary metabolite genes. As the Streptomycenstakes 7-10 days to grow in the incubator, microbes were isolated from 4 terrestrial samples in order to do more work in the lab during the incubation time. On the whole, 39 strains of Streptomyceswere chosen from ACM, 9 of them did not grow. This could be because the media used did not suit them given that only ISP-2 was used for the initial cultivation. Nonetheless, some of them exhibited remarkable results with the activation studies and interesting chemical components with optimisation.
The isolation of microbes obtained from terrestrial soil samples is of major importance since it helps in identifying new species of microbes. These experiments are important in identifying known and unknown microbes which can be utilized for further studying to isolate vital natural products. In this study, it has been established that Streptomyces spp. contains secondary metabolite genes that are not transcriptionally activated under cultivation conditions, but that can be activated by natural chemical and biological cues such as tuberculosis (TB) cells, mycolic acid and lipopolysaccharide (LPS). This study is significant since the activation can be used to produce antibiotics which are vital in the pharmaceutical industry.
1 Introduction:
Natural products have had a close association with medicine since the dawn of mankind, and the field of pharmacology traces its origins to the use of medicinal plants, animals and microbes (Peláez, 2006). Early civilizations used products available from nature to deal with injuries and diseases, and in so doing, often attributed a mystical quality to their world. Bioactive compounds derived from the natural world are responsible for many of the therapeutic effects that our ancestors experienced, and today, these compounds serve as the framework by which scientists develop novel drugs (Butler &Buss, 2006). It is the vastness of the variety of molecules in the natural world...
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