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5 pages/≈1375 words
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Style:
APA
Subject:
Biological & Biomedical Sciences
Type:
Lab Report
Language:
English (U.S.)
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Topic:

Osmosis effect Biological & Biomedical Sciences Lab Report

Lab Report Instructions:

lab report
-marking criteria,experiment notes and lab report template attached.


 


Osmosis effect Biological & Biomedical Sciences Lab Report


Criteria Sheet for Practical Reports

Note: Descriptions refer to the minimum requirements of each grade. 4 3 2 1 0

Hypothesis Hypothesis is both testable and falsifiable based on the materials available, and is clearly written Hypothesis is both testable and falsifiable based on the materials available, but has minor problems with expression and clarity Hypothesis is only either testable or falsifiable based on the materials available, but is well written Hypothesis is only either testable or falsifiable based on the materials available, and has some problems with expression and clarity Hypothesis is neither testable nor falsifiable based on the materials available, and has major problems with expression

Methods Methods are integrated in complete sentences, and written in the past tense Methods are integrated in bullet points, but written in past tense Methods are integrated in bullet points, and written in wrong tense Methods are not integrated, and presented in wrong tense Methods are not presented All details which influenced the results are included, with no trivial details All details which influenced the results are included, along with some trivial details Most details of experimental parameters are included, and/or some trivial details Some important experimental details are missing, and/or numerous trivial details included Materials and Methods did not include any experimental detail Design included valid control samples, and are clearly specified Design included valid control samples, but are not clearly specified Design included control samples, some of which are not valid Design included control samples, but are neither valid nor specified Design did not include any control samples

Results Text clearly describes important relationships between the tested variables, summarising each figure and table Text describes general trends in the data, but misses highlighting some important points Text describes the trends in data but the data was wrongly interpreted Text adds nothing to interpretation of the tested variables No description of results in text format All figures present variables on correct axes, with labels and units Figures contain errors in either axes labels or units Figures contain errors in both axes labels and units Figures lack axes labels and units No Figures presented All figure legends contain brief descriptions of the data & experimental conditions, and how it was presented All figure legends contain brief descriptions of the data & experimental conditions, but did not include details on how it was presented Some figure legends lack important details of the data and/or its presentation Most figure legends lack important details of the data and its presentation Figure legends contain no descriptions of what the data were

Discussion Answers consistently reflect a deep understanding of the biology involved Most answers reflect a good understanding of the biology involved Answers show some misunderstandings of the biology involved Answers show major misunderstandings of the biology involved Answers reflect no knowledge of the biology involved All trends in results are explained as specific changes in the biological mechanisms Most trends in results are explained as specific changes in the biological mechanisms Some links to the biological mechanisms are made during result analysis Incorrect biological reasons are given for the trends in the results No biological reasons are given for the trends in the results Results are consistently used Results are most of the time Results are occasionally used Results are never used as Results are not referred to in as evidence to support claims used as evidence to support claims as evidence to support claims evidence to support claims discussion In text citations are used to support major claims, and a reference list is provided In text citations are used to support major claims, but with minor errors in citations and/or reference list Some references are used in the discussion, with important errors in citations and/or referencing list Invalid references are used, and/or a reference list is not provided No evidence of having used reference material Writing All answers have clear meaning, logical flow, and are complete sentences Most answers have clear meaning, logical flow, and are complete sentences Writing has some problems in clarity, flow of ideas, grammar or spelling Writing has major problems in clarity, flow of ideas, grammar or spelling Answers are neither provided nor had clear meaning

Lab Report Sample Content Preview:

Lab Report
Name
Institutional affiliation
Course
Instructor
Date
Case Study
You are a veterinarian working in Indooroopilly and return from lunch to find a nurse treating a six-year-old kelpie, Baxter. Baxter is very lethargic, has an increased heart rate, and when you pinch his skin, the fold remains visible. The owner tells you that they knew something was wrong when, upon returning to the outdoor car park from a three-hour shopping trip, they saw Baxter passed out on the back seat. The temperature in the car would have been very high, and Baxter will have lost a lot of water through evaporation as he panted to stay cool. This loss of water would have reduced his blood volume, thereby increasing the concentration of the ECF.
Hypothesis
This is the hypothesis that you developed before conducting your experiment.
It is hypothesized that a hypertonic solution will cause a decrease in the PCV when compared to the negative control, which is the isotonic solution (150nM). This experiment is done in Sheep (Ovis aries) red blood cells.

Materials and Methods
1M stock solution of Sodium Chloride was added in a 100-ml volumetric flask. After this process, micro-centrifuge, there was the labeling of test-tubes, as well as hematocrit tubes depending on the tests that were to be carried. After correctly labeling the test tube, a pipette was used to add one millimeter of deionized H2O into the first test-tube. As a result, the solution resulted in an isotonic solution, which was marketed as negative control 0 M. following this activity, I decreased the volume of water in the test tube by 0.1milimeters. The experiment was proceeded by filling the test tube to provide a stock solution, which was then used in raising the volume of the test tube to 2millimters. After this, there was the labeling of the test tube that was with the 0.1-millimeter solution as 0.05M. the concentrations that were obtained included 0, 0.05, 0.1, 0.15, 0.2, and 0.25. this was followed by using ratio 1:3 to pipette blood and solutions into the micro-centrifuge, which had been set before. The main method of combining the solutions was by inversion of the tubes in a gentle measure. After this, the solutions of blood were then put into hematocrit tubes, which were then plugged by the use of plasticines. It was followed by carefully placing the hematocrit tubes into centrifuges, ensuring that a state of uniformity was realized. The hematocrit centrifuge was then to spin for 3minutes at 10000RPM. Once there was the completion of this process, the PCV result was obtained by the use of the Critocap meter. There was conducting of comparative analysis, and the results were put into graphs

lecnotes-compare your data with other groups that you did this put in method question,past tense, don't want list only paragraph form, add negative and positive control
Preparation of Stock NaCl centrifuge settings
1M Stock solution = 5.84 g of NaCl dissolved in 100 ml of H2O
Working Solutions – Dilution Calculations
Table 1: Calculations for NaCl working solutions

C1
Concentration of Stock

V1
Added Volume of Stock

C2
Desired Dilution Concentration

V2
Required Total Final Volume

(mM)...
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