Lab Report On Chromatogram Result
Abstract (15 marks)
• Typically not longer than a paragraph (avoid lengthy introductory statements and discussions).
• Must briefly state the main results;
• no citations should be included;
• should state a brief research hypothesis and indicate if results support hypothesis or not.
Give a general background of the topic and its relevance (“why is this important?”);
Introduction
• must include a minimum of 2 different citations.
• Provide a clear research hypothesis, but only at the end of the introduction.
Results
• Must include a narrative. Write in clear sentences what your results were, and include illustrations (photos) from the labs; if appropriate, include tables.
Discussion
• Interpretation of results obtained.
• Must include citations.
• Literature citations must be used to interpret your results. A discussion is a comparison of your results with results from the literature (citations).
• Indicate if your experimental results confirmed your hypothesis. If they don’t, come up with possible explanations why they didn’t.
• Results must be explained, not just repeated. Why it is important to understand this information? What are possible sources of experimental error?
Lab Report on Chromatogram Result
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Abstract
The paper chromatography is the absorbent and filter paper where the different degrees of solubility in the solvents (methanol) allow separation when a solution thereof ascends by capillarity through a strip of chromatography paper in the solvent. The most soluble pigments will move faster and rise, while the less soluble pigments, advanced less in the paper strip. Bands of different colors indicating the pigments appeared, and from the top to bottom they were carotene, followed by chlorophyll a, then chlorophyll b, and lutein. Paper chromatography was useful in the separation of plant pigments and identifying these pigments.
Introduction
Chloroplasts have a mixture of pigments with different colors: chlorophyll-a (blue-green), chlorophyll-b (yellow-green), carotene (orange) and lutein (yellow) where lutein is a xanthophyll. Separation of photosynthetic pigments using paper chromatography is one of the common approaches (Barden-Gabbei & Moffitt, 2006). In the experiment, the aim is to extract these pigments, the products, as well as identify them. Methanol is the solvent and a comparison of the distance traveled by the various substances, and the solvent helps to determine the plant pigments.
Generally, the safety precautions are observed when handling methanol, and the green color of the leaves was contained after grinding in a test tube from where the pigment solution was collected. The strips of chromatography paper were cut and drops of pigment solution placed and spaced in time (Bettelheim & Landesberg, 2013). The test tube was added to the test tube and the chromatographies placed there to achieve separation. The pigment degradation was observed and recorded.
Hypothesis: The chromatography paper is useful to separate mixed compounds and identify the pigments
Results
In the solution extracted from the plant, there were different pigments as colored ba...
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