Cell Culture

research proposal on NGF I WILL PROVIDE A DATA SHEET AND the graph information and what to do in the assignment and how to do the graph
How to do the graph: You will need two figures for your final paper - the amount of differentiation, and the amount of proliferation in response to NGF over time. If you are very familiar with creating graphs and adding error bars using Excel (or another suitable program), use this chapter as a guide as to what is expected for the Results section in your final paper. If you wish, you can come to this lab with the work complete, have it checked, and be on your way.
For differentiation, you will plot the percent of differentiated cells in each condition on the y axis, with time on the x axis. All concentrations will go on one graph, with a legend to identify each line. Error bars on each data point will indicate the standard error above and below the mean. A very important point to note is that the default in Excel is to put the same amount of error on each data point in a series, but if you do this for your paper, you will not be able to interpret your data correctly. There will be a step by step hand out in the lab to guide you through the process and we will help with any parts that are not clear. If you plan to work on your own, you can check out this tutorial.
If you are not so familiar with Excel, or you would simply like to create your graphs with people around to help, bring your laptop and we will work through it together. We will have laptops you can borrow, and there will be lots of time for questions.
maxim-hopman-fiXLQXAhCfk-unsplash.jpg
[1] Image courtesy of Maxim Hopman, on Unsplash
You will be provided data for second graph, for proliferation, and it will be much the same, with number of cells on the y axis (plotted as the number X 106) and time on the x axis. You will also be provided with data on survival, which can be summarized in the text. For survival data, instead of referencing a figure, you can add "(data not shown)" after first mention.
Before the lab, you may want to review the results section at http://urbiolabreports(dot)wikidot(dot)com/results. (We will not be going over this in the lab, although if you have questions, we can discuss them.) There is a lot of information here, and an example of a self-contained graph.
Once the visual parts of your results are complete, you can begin the text portion. The text and the visuals should form a coherent presentation of the data you obtained. Your goal is the summarize the trends you see in the graph, so do not repeat details if the information is clearly visible on the graph. The written portion need not be lengthy. Anything you summarize in the results text must be addressed in your Discussion.
Always introduce figures in the text before you show them to your reader. For example, your text may read: "Differentiation peaked in the 100 ng/ml condition on the third day (Fig. 1)." Insert your Figure 1 into the text at the end of this paragraph.
One last point about graphs. In the past, students have used various ways to try to show trends on graphs. A wise U or R University professor says, “By default, there should be straight lines connecting the points in the graph. If a curve-fitting program is used, the equation used to fit the curve should be specified, and there must be a good reason to use that equation (e.g. the Michaelis-Menten equation is logical for enzyme kinetics). The nice curves fit by Excel look good, but they have no biological meaning."
Interpreting Error Bars
NEED TO RE-WORK THIS PART DEPENDENT ON WHAT I GIVE THEM
When you interpret visuals with error bars, note that whether or not you will be able to draw statistical conclusions will depend of the number of replicates you used. If you used less than 6 replicates (two independent data points from three independent experiments), you cannot reliably use error bars to draw conclusions regarding statistical significance. This will be the case for your data, because whereas you gathered 6 data points for each concentration for each point in time, these were all generated by one experiment, i.e. we did not have three independent experiments. However, you will be able to examine the error bars to draw conclusions regarding possible trends that could be confirmed or rejected if you were to repeat the experiment at least twice more, based on the following points:
if two error bars do not overlap, you can suggest that more robust data could reveal statistical difference, and vice versa
if an error bar is small (e.g. <10% total) you can conclude that the data is of reasonable quality based on this lack of variation in the samples, and vice versaAlthough scientific papers are always presented in the same format, they are rarely written in that order. The following includes suggestions on how to write your paper so that it will be complete and coherent. Read through it completely once to give you the overall picture, then follow it through paying more attention to the details.
what to do in graph: The second section of this assignment does not have to be written in paragraph form; in fact, it may be easier and clearer to use some combination of point form, tables, flow charts, or figures. You need to work on this as a group, but everyone should include it in their assignment. What follows will give you the information you need, and there is a checklist at the end so that you can make sure you have included everything. The day you plate cells during your lab time, Day 1, is included for clarity, but you do not need to include it in the outline.
The rationale should be written in paragraph form, and be no more than 600 words, excluding references. Do not include a cover page or headers and footers, but do include page numbers. Use 10 pt. Arial font with normal margins. Just before you finish, check that you have referenced properly in the text and at the end. The guidelines for referencing are described at http://urbiolabreports(dot)wikidot(dot)com/references. This will be the referencing format you will use for your final paper, so this is a chance to start becoming familiar with it. Remember that you need at least 3 references from the primary literature.
i will provide u rest information giving my id password